Team:Membrane Biochemistry and Transport
Institut Pasteur, 28 rue du Dr Roux, 75015 Paris
Team Leader : Thomas Wollert, Research Director (email@example.com)
Team members 2022 :
Stéphane Blanchard, research technician
Daniele Campisi, postdoc
Christine Girard-Blanc, research assistant
Jagan Mohan, postdoc
Sophie Murtici, administrative assistant
Puck Norell, PhD student
Saloni Partel, postdoc
Sowmya Rama, postdoc
From left to right : Stéphane Blanchard, Gwendal Guerin (étudiant), Saloni Patel, Sowmya Rama, Christine Girard-Blanc, Jagan Mohan, Sophie Mutrici.
Keywords: Autophagy, ATG8, neurodegenerative diseases, Alzheimer’s disease, in vitro reconstitution
Research Topic :
We are investigating autophagy, which is the most versatile recycling machinery in human cells. Autophagy sequesters cytoplasmic material in a newly formed membrane vesicle, called autophagosome, and transports it to lysosomes. A major function of autophagy is to maintain cellular homeostasis by degrading protein aggregates or non-functional organelles which are potentially cytotoxic. However, starvation and toxic insults elicit another mode of action. Under these conditions, autophagy does not select cytoplasmic material for degradation but sequesters bulk cytoplasm. What sounds like wasting resources is indeed essential for cells to survive those conditions. Although the cell sacrifices functional proteins, organelles and ribosomes, it replenishes building blocks that are needed to maintain the most essential vital functions of human cells. What happens if autophgy fails a major research focus of our team.
How does it work? This is a very simple question but the answer has puzzled many research teams over the past decades. Here is what we know: Ground-breaking work by many teams worldwide revealed that during autophagy, a membrane sac (or cistern) is formed, which acts like a “waste bag” and captures either distinct cytoplasmic components or bulk cytoplasm. Expanding of the structure sequesters autophagic cargo entirely and allows the membrane to finally close upon itself. The resulting double-membrane vesicle – the autophagosome is transported to lysosomes where autophagic cargo is degraded. This process is driven by a dedicated machinery comprising more than forty autophgy related (ATG) proteins. These proteins are organised in functional units (complexes) that are sequentially recruited to the site of phagophore formation. The endoplasmic reticulum serves as a platform from which phagophores emerge. The process involves remodelling of the ER into a specialised domain, called omegasome. Early ATG complexes recruit membrane vesicles to this ER-domain and their fusion generates a membrane precursor at which downstream ATG complexes assemble. Expansion of this precursor allows cargo or bulk cytoplasm to be sequestered. The lipids that are required for this expansion are donated by vesicles which are originating from various sources as well as through direct transfer of phospholipids from the ER. Sealing of the phagophore gives rise to an autophagsome that fuses with lysosomes to expose its cargo to lysosomal hydrolyses. But what happens if autophagy does not work? The onset of several human diseases including cancer, autoimmune and metabolic diseases, as well as neurodegeneration correlates with a reduction in autophagy. Although the involved molecular mechanisms are not well understood, the process is age-dependent which increases the risk to develop related disease in older individuals. Slowly or non-dividing cells such as neurons are thus particularly vulnerable. We recently identified a specialised autophagy pathway that degrades protein aggregates in neural cells.
Impaired degradation of protein aggregates in neural cells correlates with a reduced expression of TECPR1. We identified an autophagy pathway in neural cells that promotes degradation of protein aggregates. Protein aggregates are enclosed by autophagosomes that are decorated with a specific marker, LC3C. The lysosomal protein TECPR1 binds LC3C to promote fusion of autophagosomes and lysosomes. Impaired autophagy leads to an accumulation of toxic protein aggregates and damage cells over time.
Wetzel, L., Blanchard, S., Rama, S., Beier, V., Kaufmann, A. & Wollert, T. (2020) TECPR1 promotes aggrephagy by direct recruitment of LC3C autophagosomes to lysosomes. Nat. Commun. 11, 2993.
Matscheko N, Mayrhofer P, Rao Y, Beier V & Wollert T. (2019) Atg11 tethers Atg9 vesicles to initiate selective autophagy. PLoS Biol. 29;17(7):e3000377.
Moparthi, S.B. & Wollert, T. (2018) Reconstruction of destruction – reconstitution methods in autophagy research, J. Cell. Sci., 132 (4).
Rao, Y., Perna, M.G., Hofmann, B., Beier, V. & Wollert, T. (2016) The Atg1-kinase complex tethers Atg9-vesicles to initiate autophagy. Nat. Commun., 12 (7), 10338. doi: 10.1038/ncomms10338.
Kaufmann, A., Beier, V., Franquelim, H.G. & Wollert, T. (2014) Molecular Mechanism of autophagic membrane-scaffold assembly and disassembly. Cell, 156, 469-481.
Wollert, T. & Hurley, J. H. (2010). Molecular Mechanism for Multivesicular Body Biogenesis by the ESCRT Complexes. Nature, 464, 864-869.
Wollert, T., Wunder, C., Lippincott-Schwartz, J. & Hurley, J. H. (2009). Membrane scission by the ESCRT-III complex. Nature, 458, 172-177.
Wollert, T., Pasche, B., Rochon, M., Deppenmeier, S., van den Heuvel, J., Gruber, A.D., Heinz, D.W., Lengeling, A. & Schubert, W.-D. (2007). Extending the host range of Listeria monocytogenes by rational protein design. Cell, 129, 801-902.