Research Fellow affiliated with the project Nuclear roles of members of the ATG8 family of autophagy proteins
Terje Johansen Lab, Molecular Cancer Research Group,Department of Medical Biology, Faculty of Health Sciences, Tromsø, Norway
Deadline: Saturday, December 1, 2018.
Starting date: January 1, 2019 (4 years)
Website: click here
The successful applicant is going to work on the project entitled “Nuclear roles of members of the ATG8 family of autophagy proteins “ funded by UiT – The Arctic University of Norway. The aim of this project is to elucidate the the nuclear roles of LC3B and GABARAPL2. The activity of the autophagy process may be regulated by nuclear sequestration of LC3B or other ATG8s. We will test how this regulation occurs and the impact this has on the autophagy process. ATG8 family proteins are involved in selective degradation of nuclear substrates. LC3B binds to the nuclear lamina protein lamin B1 and mediates nucleus-to-cytoplasm transport that delivers lamin B1 to the lysosome for degradation upon expression of activated RAS oncogene. This new function of autophagy acts as a guarding mechanism protecting cells from tumorigenesis. We will look for more such nuclear substrates of autophagy. The student will in parallel analyze LC3B and GABARAPL2 in ATG8 KO cells (already made) reconstituted with WT and mutants of LC3B and GABARAPL2. Live cell imaging of tagged proteins and various established assay to monitor autophagy will be used to test candidates for nuclear substrates for selective autophagy.
The successful applicant must teach at the Department’s various courses. The duties will be allocated
based on the applicant’s qualifications and the needs of the Department, and may include exam work, leading seminars, and leading or assisting in lab courses.Tweet